Based on in silico and functional analyses, the strongest gene target candidates will be prioritized in future vaccine development efforts to prevent future pasteurellosis outbreaks.Over a thousand nucleus-encoded mitochondrial proteins are imported from the cytoplasm; however, mitochondrial (mt) DNA encodes for a small number of critical proteins and the entire suite of mttRNAs responsible for translating these proteins. Mitochondrial RNase P (mtRNase P) is a three-protein complex responsible for cleaving and processing the 5'-end of mttRNAs. Mutations in any of the three proteins can cause mitochondrial disease, as well as mutations in mitochondrial DNA. Great strides have been made in understanding the enzymology of mtRNase P; however, how the loss of each protein causes mitochondrial dysfunction and abnormal mttRNA processing in vivo has not been examined in detail. Here, we used Drosophila genetics to selectively remove each member of the complex in order to assess their specific contributions to mttRNA cleavage. Using this powerful model, we find differential effects on cleavage depending on which complex member is lost and which mttRNA is being processed. These data revealed in vivo subtleties of mtRNase P function that could improve understanding of human diseases.Despite advances in medicine, cardiac disease remains an increasing health problem associated with a high mortality rate. Maladaptive cardiac remodeling, such as cardiac hypertrophy and fibrosis, is a risk factor for heart failure; therefore, it is critical to identify new therapeutic targets. Failing heart is reported to be associated with hyper-ubiquitylation and impairment of the ubiquitin-proteasome system, indicating an importance of ubiquitylation in the development of cardiac disease. Ubiquitylation is a post-translational modification that plays a pivotal role in protein function and degradation. In 1995, homologous to E6AP C-terminus (HECT) type E3 ligases were discovered. E3 ligases are key enzymes in ubiquitylation and are classified into three families really interesting new genes (RING), HECT, and RING-between-RINGs (RBRs). Moreover, 28 HECT-type E3 ligases have been identified in human beings. It is well conserved in evolution and is characterized by the direct attachment of ubiquitin to substrates. HECT-type E3 ligase is reported to be involved in a wide range of human diseases and health. The role of HECT-type E3 ligases in the development of cardiac diseases has been uncovered in the last decade. There are only a few review articles summarizing recent advancements regarding HECT-type E3 ligase in the field of cardiac disease. This study focused on cardiac remodeling and described the role of HECT-type E3 ligases in the development of cardiac disease. Moreover, this study revealed that the current knowledge could be exploited for the development of new clinical therapies.Geothermal process equipment and accessories are usually manufactured from low-alloy steels which offer affordability but increase the susceptibility of the materials to corrosion. Applying erosion-corrosion-resistant coatings to these components could represent an economical solution to the problem. In this work, testing of two newly developed laser metal deposited high-entropy alloy (LMD-HEA) coatings-CoCrFeNiMo0.85 and Al0.5CoCrFeNi, applied to carbon and stainless steels-was carried out at the Hellisheidi geothermal power plant. Tests in three different geothermal environments were performed at the Hellisheidi site wellhead test at 194 °C and 14 bar, erosion test at 198 °C and 15 bar, and aerated test at 90 °C and 1 bar. Post-test microstructural characterization was performed via Scanning Eletron Microscope (SEM), Back-Scattered Electrons analysis (BSE), Energy Dispersive X-ray Spectroscopy (EDS), optical microscopy, and optical profilometry while erosion assessment was carried out using an image and chemical analysis. Both the CoCrFeNiMo0.85 and Al0.5CoCrFeNi coatings showed manufacturing defects (cracks) and were prone to corrosion damage. Results show that damage in the CoCrFeNiMo0.85-coated carbon steel can be induced by manufacturing defects in the coating. This was further confirmed by the excellent corrosion resistance performance of the CoCrFeNiMo0.85 coating deposited onto stainless steel, where no manufacturing cracks were observed. Indonesia ranks 7th highest in the world for the number of deaths caused by tobacco use including those caused by Chronic Obstructive Pulmonary Disease (COPD). The purpose of this study was to determine the influence of initial smoking age and habit on the incidence of COPD. This research was a case-control study. The sampling in this research took a systematic random sampling method. The samples of this study were 56 respondents of a case group and 56 respondents of a control group. This study was conducted at Ngudi Waluyo Hospital, Wlingi, Blitar from October to November 2017. The factors that influenced the incidence of COPD were being male ( = 0.00; OR = 6.333; 95%CI = 2.776-14.450), a smoker ( = 0.00050; OR = 5.1318; 95%CI = 1.9004-13.8958), initially smoking at <15 years old ( = 0.00; OR = 11,769; 95%CI = 4.086-33.903), initially getting into a smoking habit at the age of <15 years old (OR = 12; CI = 1346-106,950), initially getting into a smoking habit at the age of ≥15 years old (OR = 3647; CI = 1625-8183) and having smoked for ≥30 years (OR = 8857; CI = 3298-23,787). There are three factors of smoking behavior that influence COPD smoking habit, initial smoking age and smoking duration. Of all factors, forming a smoking habit at the age of <15 years old has the biggest risk (OR = 12; CI = 1346-106,950).There are three factors of smoking behavior that influence COPD smoking habit, initial smoking age and smoking duration. Of all factors, forming a smoking habit at the age of less then 15 years old has the biggest risk (OR = 12; CI = 1346-106,950).This study aimed to evaluate two hydrogen peroxide (HP)-based at-home bleaching systems in order to analyze whether nano-hydroxyapatite (nHA) addition may represent a reliable and safe solution for tooth whitening without altering dental microstructure and hardness. Human third molars (N = 15) were treated with two bleaching agents, one containing 6%HP (6HP) and the other 6% HP nHA-enriched (6HP-nHA) with average particle diameter ranging from 5-20 nm. Their effects on enamel were assessed using a spectrophotometer, Vickers microhardness (VMH) test and Scanning Electron Microscopy (SEM), comparing the treated groups with the non-treated control group (CTR). see more Color analysis revealed improvement in whiteness in both groups compared to CTR. VMH test results showed no differences among the groups. SEM analysis highlighted no evident changes in the enamel microstructure of tested groups compared to CTR. At high magnification, in 6HP group, a slight increase in irregularities of enamel surface morphology was observed, while 6HP-nHA group displayed removal of the aprismatic layer but preservation of the intact prismatic structure.